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Multiplex Immunofluorescence(mIF) Kit

The Multiplex Immunofluorescence(mIF) technology allows for the simultaneous labeling of multiple biomarkers on a single tissue section, enabling comprehensive analysis of the tumor immune microenvironment, quantitative evaluation of cell phenotypes, and detailed understanding of cell interactions and spatial relationships. This is achieved using tyramide signal amplification (TSA) technology with horseradish peroxidase (HRP) and a proprietary Microstacker Polymer.

Product Feature

Multiplex Immunofluorescence (mIF) is an advanced technique widely used in pathology and biomedical research to simultaneously detect and visualize multiple biomarkers within a single tissue section. It enables detailed analysis of protein expression, interactions, and spatial distribution within the tissue microenvironment.

– Allows protocol runs onboard with CNT320/330/360

– 4-8 markers detection simultaneously

– No species restrictions

– High Sensitivity and Specificity

Specification

Cat#	Product name	Format	Components
FM2007	7-color multiplex fluorescence IHC detection kit	20T / 50T	Endogenous Peroxidase Blocking reagent Anti-Mouse/Rabbit HRP-Polymer TSA Fluorophore CM480 (Ex:425nm, Em:485nm) TSA Fluorophore CM520 (Ex:490nm, Em:520nm) TSA Fluorophore CM570 (Ex:550nm, Em:570nm) TSA Fluorophore CM620 (Ex:585nm, Em:615nm) TSA Fluorophore CM690 (Ex:675nm, Em:690nm) TSAFluorophore CM780 (Ex:750nm, Em:770nm) Spectral DAPI concentrate (Ex:355nm, Em:460nm) TSA-biotin Amplification Diluent PBS Buffer
FM2004	4-color multiplex fluorescence IHC detection kit	20T / 50T	Endogenous Peroxidase Blocking reagent Anti-Mouse/Rabbit HRP-Polymer TSA Fluorophore CM520 (Ex:490nm, Em:520nm) TSA Fluorophore CM570 (Ex:550nm, Em:570nm) TSA Fluorophore CM690 (Ex:675nm, Em:690nm) Spectral DAPI concentrate (Ex:355nm, Em:460nm) Amplification Diluent
FM2017	7-color multiplex fluorescence IHC detection kit (rabbit-on-rodent)	20T / 50T	Endogenous Peroxidase Blocking reagent Anti-Rabbit HRP-Polymer TSA Fluorophore CM480 (Ex:425nm, Em:485nm) TSA Fluorophore CM520 (Ex:490nm, Em:520nm) TSA Fluorophore CM570 (Ex:550nm, Em:570nm) TSA Fluorophore CM620 (Ex:585nm, Em:615nm) TSA Fluorophore CM690 (Ex:675nm, Em:690nm) TSAFluorophore CM780 (Ex:750nm, Em:770nm) Spectral DAPI concentrate (Ex:355nm, Em:460nm) TSA-biotin Amplification Diluent PBS Buffer
FM2014	4-color multiplex fluorescence IHC detection kit (rabbit-on-rodent)	20T / 50T	Endogenous Peroxidase Blocking reagent Anti-Rabbit HRP-Polymer TSA Fluorophore CM520 (Ex:490nm, Em:520nm) TSA Fluorophore CM570 (Ex:550nm, Em:570nm) TSA Fluorophore CM690 (Ex:675nm, Em:690nm) Spectral DAPI concentrate (Ex:355nm, Em:460nm) Amplification Diluent
FT480	TSA Fluorophore CM480	20T / 50T	TSA Fluorophore CM480 (Ex:425nm, Em:485nm), Amplification Diluent
FT520	TSA Fluorophore CM520	20T / 50T	TSA Fluorophore CM520 (Ex:490nm, Em:520nm), Amplification Diluent
FT570	TSA Fluorophore CM570	20T / 50T	TSA Fluorophore CM570 (Ex:550nm, Em:570nm), Amplification Diluent
FT620	TSA Fluorophore CM620	20T / 50T	TSA Fluorophore CM620 (Ex:585nm, Em:615nm), Amplification Diluent
FT690	TSA Fluorophore CM690	20T / 50T	TSA Fluorophore CM690 (Ex:675nm, Em:690nm), Amplification Diluent
FT780	TSA Fluorophore CM780	20T / 50T	TSAFluorophore CM780 (Ex:750nm, Em:770nm) Amplification Diluent TSA-biotin PBS buffer
FTDAPI	Spectral DAPI concentrate	20T / 50T	Spectral DAPI concentrate (Ex:355nm, Em:460nm)

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Specification

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